Protein Analysis Reagents
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Thermo Scientific™ Pierce™ Premium Grade DSP (dithiobis(succinimidyl propionate))
Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this popular amine-reactive, cleavable crosslinker.
| Shipping Condition | Ambient |
|---|---|
| Content And Storage | Upon receipt store desiccated at 4°C. |
| Cleavable | By Thiols |
| Water Soluble | No |
| Form | Powder |
| Molecular Weight (g/mol) | 404.42 |
| Solubility | DMF, DMSO |
| Labeling Method | Chemical Labeling |
| Crosslinker Type | Homobifunctional |
| Chemical Reactivity | Amine-Amine |
| Cell Permeability | Yes |
| Format | Standard, Single-use, Premium-grade |
| Product Type | Crosslinker |
| Spacer | Medium (10 to 30 Å) |
| Formulation | Dithiobis(succinimidylpropionate); powder solid |
| Reactive Moiety | NHS Ester |
| Spacer Arm Length | 12.0 Å |
| Product Line | Pierce |
| PEGylated | No |
Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit
Obtain bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of red firefly luciferase reporters
| Substrate | D-Luciferin |
|---|---|
| Assay | Reporter Enzyme, Luciferase Reporter Assay |
| Substrate Properties | Chemical Substrate |
| Target | Luciferase, Firefly Luciferase |
| Type | Assay Kit |
| Format | 384-well plate, 96-well plate |
| For Use With (Equipment) | Luminometer (Microplate) |
| Compatible Cells | Mammalian Cells |
| Substrate Type | Luciferase Substrate |
| Technique | Enhanced Chemiluminescence |
| Label Type | Enzyme Labeled |
| Product Line | Pierce |
| Detection Method | Bioluminescence |
Thermo Scientific™ Pierce Protease Inhibitor Tablets
Thermo Scientific™ Pierce Protease Inhibitor Tablets and Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments.
Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format
Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.
| Cleavable | No |
|---|---|
| PEGylated | No |
Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
| Content And Storage | Store desiccated at -20°C, protect from moisture |
|---|---|
| Product Type | NHS-Fluorescein |
| Label or Dye | FITC (Fluorescein) |
| Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
| Solubility | DMF (Dimethylformamide) |
| Emission | 518 |
| Excitation | 494 |
| Label Type | Classic Dyes |
| Chemical Reactivity | Amine |
| Product Line | Pierce |
Thermo Scientific™ Guardian™ Peroxidase Conjugate Stabilizer/Diluent
Preserve diluted (1-1,000ng/mL) HRP conjugates for more than six months at room temperature without significant loss of activity using Guardian Peroxidase Conjugate Stabilizer/Diluent.
| Physical Form | Liquid |
|---|---|
| Chemical Name or Material | Peroxidase Conjugate Stabilizer/Diluent |
| Recommended Storage | Upon receipt store at room temperature or 4°C. |
| Product Line | Guardian |
Thermo Scientific™ HisPur™ Cobalt Superflow Agarose
Perform high-purity, large-scale purification of polyhistidine-tagged proteins with this tetradentate-chelating Superflow 6 support charged with divalent cobalt.
| Stationary Phase | Metal Chelate, Divalent Cobalt |
|---|---|
| Type | Agarose |
| Target | His-Tagged Protein |
| Content And Storage | Store at 4°C |
| Format | Bottle |
| Form | Liquid Suspension |
| Column Type | Affinity, Agarose Resin |
| Product Line | HisPur |
Molecular Probes™ Alexa Fluor™ 488 Microscale Protein Labeling Kit
Microscale Protein Labeling Kits provide a convenient means for attaching a fluorescent label to a small amount of antibody or protein (20–100 μg).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ Pierce™ NHS-Activated Agarose Slurry
N-Hydroxysuccinimide ester resin and columns to covalently immobilize proteins, peptides and other amine-containing ligands for affinity purification.
Invitrogen™ Zenon™ LysoLight™ Green IgG Labeling Reagents
Streamline antibody-drug conjugate screening with Zenon LysoLight Green IgG labeling reagents for the monitoring of antibody internalization. These reagents offer rapid, scalable antibody labeling and a bright fluorescence-based indication of antibody uptake and lysosomal trafficking in target cells when analyzed by flow cytometry, fluorescence microscopy, or high-content screening.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Excitation/Emission | 488/525 nm |
|---|---|
| Labeling Target | Antibodies |
| Flow Cytometer Laser Lines | Blue |
| For Use With (Equipment) | Flow Cytometer, Fluorescence Microscope, High Content Analysis Instrument |
| Product Type | Labeling Reagent |
| Spacer | None |
| Color | Green |
| Label or Dye | LysoLight Green |
| Solubility | Aqueous Buffer |
| For Use With (Application) | To screen for antibody internalization |
| Product Line | LysoLight |
| Detection Method | Fluorescence |
Thermo Scientific™ Streptavidin Protein, HRP
Thermo Scientific™ Pierce™ Streptavidin, Horseradish Peroxidase Conjugate includes streptavidin in a purified form conjugated to peroxidase for substrate-based detection.
| Shipping Condition | Ambient Temperature |
|---|---|
| Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C. |
| Conjugate | HRP |
| Target Molecule | Biotin |
| Product Type | Streptavidin Conjugate |
| Form | Solid |
| Label or Dye | HRP (Horseradish Peroxidase) |
| Product Line | Pierce |
Invitrogen™ DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)
DTNB; Ellman's Reagent, 5,5'-Dithiobis-(2-Nitrobenzoic Acid)
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific™ NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer
Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye.
Invitrogen™ Zip Alexa Fluor™ Rapid Antibody Labeling Kits
Invitrogen Zip Alexa Fluor Rapid Antibody Labeling kits allow you to efficiently and quickly label your precious antibody with Alexa Flour dyes to create an antibody conjugate that is ready to use within 15 minutes with 100% recovery.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Labeling Target | IgG |
|---|---|
| Product Type | Labeling Kit |
| Quantity | 3 Labelings |
| Label Type | Alexa Fluor |
| Labeling Scale | 100 μg |
| Chemical Reactivity | Amine |
| Product Line | Alexa Fluor |
Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli
One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Blue/White Screening | Yes (lacZΔM15) |
| Cloning Unstable DNA | No |
| Cloning Methylated DNA | Yes (mcrA) |
| Transformation Efficiency Level | High Efficiency (>1 x 1010 cfu/μg) |
| High-throughput Compatibility | Low |
| Improves Plasmid Quality | Yes (endA1) |
| Preparing Unmethylated DNA | No |
| Plasmid | May be used for plasmids >20 kb |
| Reduces Recombination | Yes (recA13) |
| T1 Phage - Resistant (tonA) | No |
| Antibiotic Resistance Bacterial | Yes (Streptomycin) |
| Format | Tube |
| Product Type | Electrocompetent Cells |
| Species | E. coli (K12) |
| Product Line | One Shot |
| Contains F' Episome | No |